Cloning and mapping of the chloroplast DNA sequences for two messenger RNAs from mustard (Sinapis alba L.)
نویسندگان
چکیده
منابع مشابه
Cloning and mapping of the chloroplast DNA sequences for two messenger RNAs from mustard (Sinapis alba L.).
Restriction fragments of chloroplast (cp)DNA from mustard leaves were cloned in E. coli using pBR 322 as the vehicle. Cloned fragments containing the structural sequences for two polypeptides of 56,000 and 35,000 daltons were selected by a mRNA hybridization-translation procedure. The cloned mustard genes are structurally related to chloroplast genes from maize. They hybridize to the maize mRNA...
متن کاملSequence and expression characteristics of a nuclear-encoded chloroplast sigma factor from mustard (Sinapis alba).
Plant chloroplasts contain transcription factors that functionally resemble bacterial sigma factors. We have cloned the full-length cDNA from mustard (Sinapis alba) for a 53 kDa derived polypeptide that contains similarity to regions 1.2-4.2 of sigma70-type factors. The amino acid sequence at the N-terminus has characteristics of a chloroplast transit peptide. An in vitro synthesized polypeptid...
متن کاملProkaryotic features of a nucleus-encoded enzyme. cDNA sequences for chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases from mustard (Sinapis alba).
Two cDNA clones, encoding cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases (GAPDH) from mustard (Sinapis alba), have been identified and sequenced. Comparison of the deduced amino acid sequences with one another and with the GAPDH sequences from animals, yeast and bacteria demonstrates that nucleus-encoded subunit A of chloroplast GAPDH is distinct from its cytosolic counterp...
متن کاملNucleotide sequence of the intergenic spacer and the 18S ribosomal RNA gene from mustard (Sinapis alba).
From a rRNA repeating unit (9.1 kbp) of mustard we have The DNA was sequenced according to the method of Maxam sequenced the first internal transcribed spacer, the 5.8S rRNA and Gilbert gene, and the second internal transcribed spacer [1]. Here, we present the nucleotide sequence of a 5500 bp fragment containing the 3 ' end terminus of the 25S gene (486 bp), the intergenic spacer REFERENCES (32...
متن کاملMolecular Composition of Glutamine Synthetase of Sinapis alba L
Matthias Höpfner , Georg Reifferscheid, and Aloysius Wild Institut für Allgemeine Botanik der Johannes Gutenberg-Universität, Saarstraße 21, D-6500 Mainz, Bundesrepublik Deutschland Z. Naturforsch. 43c, 194-198 (1988); received November 26, 1987 Active Centers, Enzyme Purification, Enzyme Structure, Glufosinate, Glutamine Synthetase, Phosphinothricin Chloroplastic glutamine synthetase of Sinapi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1981
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/9.15.3681